FastQCFastQC Report
Sun 20 Nov 2022
CO_007-COL.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_007-COL.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46584
Sequences flagged as poor quality0
Sequence length25-347
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC21844.688304997424008No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG20684.439292460930791No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG18353.939120728147003No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG14153.037523613257771No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC13332.8614975098746354No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG13222.8378842521037266No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG13012.792804396359265No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA11772.526618581487206No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT10042.1552464365447364No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG9682.077966683839945No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT8511.8268074875493732No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG8381.7989009101837543No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG7661.6443414047741713No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC7651.642194744976816No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA7541.6185814872059077No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG7061.5155418169328525No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6891.479048600377812No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6801.4597286622016143No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT6261.3438090331444272No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA5961.2794092392237677No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG5921.2708226000343466No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG5741.232182723681951No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT5391.1570496307745148No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT5391.1570496307745148No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5361.1506096513824489No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT5021.0776232182723682No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC4941.0604499398935257No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG4931.0583032800961705No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA4891.049716640906749No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4821.034690022325262No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC4801.0303967027305512No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4400.9445303108363386No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC4310.9252103726601408No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG4260.9144770736733643No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4250.9123304138760089No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4100.8801305169156791No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCG4090.8779838571183239No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA4050.8693972179289026No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC4050.8693972179289026No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3930.8436373003606388No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3920.8414906405632836No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG3780.8114374034003091No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC3520.7556242486690709No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT3500.7513309290743603No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG3480.7470376094796497No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3440.7384509702902284No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3400.7298643311008072No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3260.6998110939378327No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG3240.6955177743431221No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2930.6289713206251073No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2550.5473982483256054No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG2370.5087583719732096No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG2280.4894384337970119No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGG2250.4829984544049459No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA2180.4679718358234587No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT2050.44006525845783956No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC1930.4143053408895758No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA1830.39283874291602267No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1780.38210544392924606No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1770.37995878413189077No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG1690.36278550575304824No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1650.354198866563627No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1650.354198866563627No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1620.34775888717156106No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC1620.34775888717156106No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1600.34346556757685043No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1600.34346556757685043No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG1590.3413189077794951No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG1570.33702558818478445No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCA1560.33487892838742916No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG1330.2855057530482569No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG1290.2769191138588356No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC1290.2769191138588356No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAG1150.24686587669586124No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGC960.20607934054611027No Hit
GCCGCCTCCCTAATGTTACACACTGACTAG820.17602610338313585No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCC680.14597286622016142No Hit
TCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT600.1287995878413189No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAG480.10303967027305513No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGA480.10303967027305513No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACCG470.10089301047569982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)